Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein.
نویسندگان
چکیده
A genetically encodable caspase-3 sensing system has been created using self-assembling split-GFP, in which a C-terminal fragment is "covalently" cyclized via a caspase-3 substrate sequence mediated by split-intein. The specific cleavage of the cyclic C-terminal fragment by caspase-3 induces the GFP reassembly and fluorescence recovery.
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ورودعنوان ژورنال:
- Chemical communications
دوره 49 87 شماره
صفحات -
تاریخ انتشار 2013